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Image Search Results
Journal: Nutrients
Article Title: Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo Clinical Trial
doi: 10.3390/nu13082653
Figure Lengend Snippet: Effect of human serum enriched with ALE metabolites on inflammatory mediator production of NO and PGE2 in primary human articular chondrocytes: Human articular chondrocytes (HACs) were harvested from the tibial plateau and femoral condyles following knee replacement surgery and isolated. HACs were preincubated with fetal calf serum for 24 h and stimulated with IL-1 β (1 ng/mL) for an additional 24 h ( A , B ). To analyze the effects of human serum enriched with metabolites, cells were preincubated for 24 h in DMEM in the presence of 10% of human enriched serum according to the Clinic’n’Cell protocol (DIRV INRA 18-00058) prior to an additional 24 h treatment with human recombinant IL-1β (Millipore Corporation, Molsheim, France) at 1 ng/mL ( C , D ). NO ( B , D ) and PGE2 ( A , C ) releases in culture media were measured. IL-1 β stimulated NO and PGE2 releases independently of the origin of the serum (calf or human). The human serum enriched with OPCO metabolites significantly limited PGE2 release but had no observable effect on NO release. * ( p < 0.05); *** ( p < 0.001); ns : no significant difference.
Article Snippet: Nitrate/Nitrite colorimetric assay and
Techniques: Isolation, Recombinant